Iosynth is best known for supplying enzymes for biocatalysis, however enzymes are also routinely used for multiple other applications. Please check back regularly as this is a continually expanding portfolio of innovative enzymes.
Proteinase K is a nonspecific serine protease with a broad substrate specificity that can be used in a wide variety of life science applications such as:
Iosynth supplies lyophilized Proteinase K in bulk quantities, up to multi kg levels.
Trypsin (EC 3.4.21.4) is an endopeptidase that preferentially hydrolyses ester bonds whose carboxyl groups are contributed by Lysine (Lys) or Arginine (Arg) except when either if followed by proline (PRO). It is used for a wide range of biotechnological processes. Although native trypsin from bovine or porcine pancreas has been available for many years, a recombinant Trypsin manufactured by microbial fermentation has many benefits.
Iosynth supplies rTrypsin as a granulated product in bulk. Before use, a filtration process of the rTrypsin in solution is required.
Beta Lactamases are a critical enzyme for antibiotic sterility testing and environmental monitoring. Eucodis Bioscience, manufacturer of the famous and trusted LacBuster brand of beta lactamase enzymes, is Iosynth’s partner in this segment.
US Pharmacopeia (USP) Chapter 71 and EP Section 2.6 describe sterility testing of bulk antibiotics, which should be shown to be free from microbial contamination. The testing requires the removal of significant amounts of active antibiotic from solution by combined filtration and the use of penicillinase or β-Lactamase, The resulting solution is tested for the (lack of) growth of microbes.
Our beta-lactamase has significantly higher activity against clinically important beta-lactams, it inactivates clinical relevant cephalosporins better than any other product available on the market.
Contact plates, settle plates and air monitoring systems for testing of aseptic conditions in antibiotic manufacturing facilities need to be manufactured with agar medium for neutralization of antibiotic. This is achieved by the addition of Penicillinase or β-Lactamase to the medium. Through this, any antibiotic residues are hydrolyzed and microbial contamination can be detected.
Our Beta-Lactam agar plates are available as contact and settle plates for high performance monitoring of your isolators and clean rooms during beta lactam manufacture.