Iosynth offers a large catalog of highly pure and active R&D enzymes for a variety of different substrates and reactions. Almost all of these enzymes are fully scalable and are available to ton scale.
Screening kits for enzymes are a quick and easy way to identify suitable enzymes for particular reaction.
Initial screening is usually done at mg level, and only promising enzyme candidates will then be taken forward.
| Screening Kit | |
|---|---|
| PROT | Protease |
| KRED / ADH | Ketoreductase / Alcohol dehydrogenase |
| ATA | ω-transaminase |
| NIT | Nitrilase |
| NHT | Nitrile Hydratase |
| AMD | Amidase |
| ERED | Ene Reductase |
| HNL | Oxynitrilase |
| NTR | Nitro Reductase |
| DERA | Aldolase |
| P450 | P450 Monooxygenase |
| AOX | Alcohol Oxidase |
| D-AADH | D-amino acid dehydrogenase |
| PLE | Pig Liver Esterase |
| EH | Epoxide Hydrolase |
| MOA | Monoamine Oxidase |
| IRED | Imine Reductase |
| NRED | Nitrile Reductase |
| PDH | Phenylalanine Dehydrogenase |
Many enzymes used for industrial biocatalysis require a cofactor for the enzyme to be catalytically active. These cofactors are not consumed but converted in the reaction. In combination with cofactor recycling enzymes, these cofactors can be reused. This combination of enzyme plus cofactor plus cofactor recycling enzyme is an efficient way of designing a biocatalytic enzyme reaction.
| COENZYMES |
|---|
| NAD |
| NADP |
| NADH |
| NADPH |
| PLP |
| NMN |
| REGENERATION ENZYMES |
|---|
| Glucose dehydrogenase (GDH) |
| Formate dehydrogenase (FDH) |
| GROUPS OF AVAILABLE ENZYMES | TYPE OF REACTION PERFORMED | ENZYME TYPE | EC NUMBER | SPECIFIC REACTION |
|---|---|---|---|---|
| Nucleoside Phosphorylases | Base exchange in a nucleoside | purine-nucleoside phosphorylase | 2.4.2.1 | Purine base exchange over nucleosides |
| pyrimidine-nucleoside phosphorylase | 2.4.2.2 | Pyrimidine base exchange over nucleosides | ||
| uridine phosphorylase | 2.4.2.3 | Pyrimidine base exchange over nucleosides | ||
| Nucleoside deoxyriboysyltransferases | - | nucleoside deoxyribosyltransferase | 2.4.2.6 | Base exchange over deoxynucleosides |
| Glycosylases | Nucleoside hydrolysis | purine nucleosidase | 3.2.2.1 | Hydrolizes a purine from a nucleoside |
| 2'-deoxynucleoside 5'phosphate N-hydrolase | 3.2.2. | Hydrolizes a base from a 2´deoxynucleoside 5´-phosphate | ||
| Deaminases | Oxidative deamination | cytidine deaminase | 3.5.4.5 | Deamination of cytidine to uridine |
| dCTP deaminase | 3.5.4.13 | Deamination of dCTP to dUTP |